Molecular Biology is a branch of biology that deals with the study of cellular components and their structure and functions. This practical manual contains 10 experimental exercises. It starts with an introduction and biochemical calculations, and continues to laboratory exercises and glossary. All exercises have been simplified in the interest of the students. The procedures of all the experiments are shown with the help of flowcharts and diagrams for more clearity. Since Molecular biology is an upcoming and fascinating branch of biological sciences, the approach of performing experiments will lead to success of learning the subject.
Experiments
• Isolation of DNA from bacterial culture
• Determination of DNA purity by Spectrophotometry method.
• Quantification of DNA by Spectrophotometry method.
• Estimation of DNA concentration by diphenylamine method.
• Agarose gel electrophoresis.
• Isolation of plasmid DNA.
• Restriction digestion of DNA.
• Amplification of DNA.
• Amplified rDNA restriction analysis (ARDRA).
• Transformation of bacterial cells.
• Glossary
Introduction
Biochemical calculation of solution
Dr. Deepak Som
Dr. Deepak Som, working as Assistant Professor in the Department of
Microbiology, Shri Guru Ram Rai University, Dehradun, Uttarakhand. He
obtained his Ph.D. degree from Pacific Academy of Higher Education and
Research, Udaipur, Rajasthan. He has teaching experience of more than 9
years. Also, he has published and presented several papers in
international and national journals and seminars.
Subject Covers:-
• Applied Research.
• Basic Research.
• Molecular Identification.
• Sequencing.
• Genetic Engineering.
This Book Useful for the students of :-
• Bio
• Chemistry
